The purpose of this study was to ascertain the prevalence of burnout and its associated risk factors among the University staff, involving both academicians and non-academicians and relate these to their job satisfaction. A cross-sectional study was conducted among the university staff involving both academicians and non-academicians. The participants were emailed the questionnaires through their university email and alternative email addresses. They were asked to complete questionnaires on their sociodemographic and work details, Copenhagen Burnout Inventory (CBI), Depression, Anxiety and Stress Scale (DASS-21) and Job Satisfaction Scale (JSS). Among the 411 participants who participated, 53% were academicians (n = 216). Academicians demonstrated greater burnout levels and psychological distress when compared to non-academicians. Correlational analyses indicated a moderate to high correlation between psychological distress and burnout due to work, personal and client where higher burnout was associated with higher psychological distress. Non-academicians demonstrated greater job satisfaction levels when compared to academicians. Correlational analyses indicated a high correlation between job satisfaction and burnout due to work, with higher burnout levels associated with lower job satisfaction among staff. This study showed that academicians suffer from high levels of burnout in aspects of personal, work and client-related matters, and this has contributed to higher psychological distress among them and significantly affect their job satisfaction.