Technological, Pedagogical, and Content Knowledge (TPACK) is a teaching framework to integrate technology in teaching. Integrating technology into the curriculum becomes an inseparable part of the best teaching practice. This study aims to produce learning tools on blended learning integrated with the PBLRQA strategy in the form of the lesson plan, evaluation sheets, and moodle-based e-learning media that are valid, practical, and effective in Animal Physiology courses. PBLRQA is an innovative learning strategy integrated between Problem-based Learning (PBL) with reading questioning answering (RQA). This study was Research and Development (R & D) using ADDIE development models (analyze, design, development, implementation, and evaluation). The validity of the product was measured using an expert validation sheet, the practicality of the product was measured using a lecturer and student response questionnaire, and the effectiveness of the product was measured using a student learning independence questionnaire. Based on the results of the study it could be concluded that the lesson plan, evaluation sheets, and moodle-based e-learning were valid, practical and effective in increasing the self-regulated learning of students. It proved that the lesson plan, evaluation sheets, and moodle-based e-learning media that integrate PBLRQA strategies are appropriate to use.