Parental Burnout (PB) is an exhaustion syndrome resulting from exposure to overwhelming parenting stress. The current gold-standard instrument, namely, Parental Burnout Assessment (PBA) was used in the International Investigation of Parental Burnout (IIPB), a 40-country study of the prevalence of PB around the world. The IIPB study has stimulated worldwide interest, but efforts are still needed to validate the PBA in different cultures. This study is the first on PB in a collectivist, predominantly Islamic country. It aims to examine the psychometric properties of the Turkish translation of the PBA. The PBA-Turkish was administered to 452 Turkish parents (60% mothers). The results showed that the PBA is a relevant construct in Turkish culture. We replicated the original four-factor structure of the PBA and tested a second-order factor structure through confirmatory factor analyses. The first- and second-order factor models fit the data well. The Emotional Distancing subscale, however, demonstrated lower reliability than the other subscales. We then attained measurement invariance across genders in a set of nested models with gradually increasing parameter constraints. Finally, non-working and single parents reported higher PB, pointing to the possible role of relationships and support as protective factors for Turkish parents.