Five standards-based science institutes (SI) were conducted between 2005 and 2016 to support improved content knowledge, pedagogy, and pedagogical content knowledge of in-service middle and high school science teachers in the area of biology. The SI were conducted for 90 hours during the summer and academic year. Content covered was related to state science standards taught in the participants' classrooms. Instruction was based on the 5E learning cycle model and emphasized the use of inquiry so that participants experienced the teaching methods specified by the science teaching standards. Pre- and post-test analysis demonstrated significant content gains using this teaching model. Teacher surveys and reflections confirmed that the program had a positive influence on implementation of classroom teaching methods and student learning. Analysis of lesson plans and classroom observations indicated that teachers were able to redesign lesson plans and teaching practices to meet state standards for incorporating inquiry into classroom teaching.