All of the behaviors that people exhibit in order to stay healthy both physically and psychologically and maintain their health during their lifetime are included in the definition of "healthy life behavior". In this study, it was aimed to identify and compare the perceptions of healthy life behavior of students of the Department of Exercise and Sport Sciences and Psychology Department Students who study at the University of Health Sciences. A total of 199 students, 97 students from the sports science department (age: 19.84 ± 1.801, weight: 65.30 ± 12.460), and 102 students from the psychology department (age: 20.12 ± 1.916 weight: 60.17 ± 9.881) participated in the study. There was a statistically significant difference between the scale total scores of the participants and the dimensions of health responsibility, physical activity, nutrition, stress management, and the measurements of the students of sports science students and students of psychology sciences in terms of an increase in sport sciences students (p < 0.05). There was no significant difference between the participants' interpersonal communication and spiritual development scores, which are the sub-dimensions of the scale (p > 0.05). University education is an education period in which students can decide independently. These results showed that sports students and psychology sciences students' different undergraduate education also affected their healthy lifestyle behaviors. In this context, it is concluded that directing students of other departments of the university to exercise and sports activities will contribute to their perceptions of healthy lifestyle.