This study aims to investigate the relationship between prospective teachers' level of performing behaviors related to learning responsibility and their level of readiness for teaching profession. The pre-service teachers (n = 408), who are in their second year at the Faculty of Education, filled in the Learning Responsibility Scale and the Readiness Scale for Teaching Profession. The Learning Responsibility Scale consists of 28 Likert-type questions and includes the subscales entitled preparation for learning, active engagement in learning, monitoring learning outcomes and enriching learning. Pearson's correlations and multiple linear regression analyzes were used to analyze the data. The results of the analysis revealed that there was a negative by moderately significant relationship between the prospective teachers' learning responsibilities and their readiness for teaching profession. In addition, it was found out that the variables of learning responsibility explained 12% of the variation in prospective teachers' scores of readiness for the profession. Active engagement in learning contributed negatively to explain the prospective teachers' readiness for teaching profession.