Metabolomics, the large-scale study of metabolites in biological fluids and tissues, offers the ability to observe metabolic changes in a broad context. Traditionally, nutritional metabolism education has focused on theory using textbooks. The objective of this study was to assess the effectiveness of involving students in a feeding research study using metabolomics analyses for the purpose of enhancing learning. The study consisted of two groups (n = 19/group) of nutrient metabolism students: (1) 12 h fasting blood draw and (2) 2 h postprandial blood draw following a breakfast meal. Plasma samples were analyzed using ultraperformance liquid chromatography tandem quadrupole mass spectrometry metabolomics assays. Pre- and postcourse questionnaires were completed by students in order to assess the educational interest in and effectiveness of the activity. The feeding intervention, sample, and data analysis were completed within the first 5 weeks of the academic quarter. The 221 metabolites identified in the plasma included energetic pathway intermediates, triglycerides, bile acids, amino acids, and acylcarnitines. Results were periodically presented in lecture and discussed using interactive group activities. Course exams included essay questions requiring explanation of observed study results. An assigned project required inclusion of study results within an integrated metabolic pathway map. Postcourse questionnaires indicated that inclusion of the feeding study enhanced learning of the relevant subject material, in addition to increasing personal investment in and excitement about the course, and knowledge of research. In conclusion, involving students as participants in research that utilizes metabolomics in university lecture courses may improve student learning of metabolism.