The aim of this research was to determine the effects of physical activity levels of teacher candidates on the sub-dimensions of health-related quality of life. In the research among the quantitative research methods, relational survey model was used. A total of 90 teacher candidates participated in this research. The International Physical Activity Questionnaire-Short Form (IPAQ-SF) and the 36-Item Short Form Health Survey (SF-36) were used in this study. In the data analysis, "percentage, frequencies, standard deviation, mean, Product-Moment Correlation coefficients and Multiple regression" were used. According to the results, role functioning/emotional, pain and general health sub-dimensions are important predictors on physical activity levels. However, physical functioning, emotional well-being, vitality, social functioning, role functioning/physical, sub-dimensions have not an important impact on physical activity levels statistically. As a conclusion, participation in physical activity can be said to have a negative effect on emotional problems and pain, and a positive effect on general health status.