In this study, we examined the effects of multiple reference pitch sources on collegiate singers' accuracy in pitch-matching and intonation tasks. We also investigated which reference pitch source participants preferred and for what reasons. Participants (N = 99) sang a two-measure excerpt of "Joseph Dearest, Joseph Mine" after listening to the starting pitch of A on a pitch pipe, the piano, a vocal hum, or a tuning fork in two conditions. For one tuning fork condition, participants' starting pitch was an A, the same pitch as the tuning fork. For the other tuning fork condition, their starting pitch was a G, a different pitch than the tuning fork. We selected two pitches for analysis, each corresponding to the first syllable of the word "Joseph." We then analyzed pitch deviation of the two target notes from the reference pitch in each condition. Participants were most accurate in response to the piano and least accurate in response to the tuning fork when their starting pitch was a G. Participants expressed preference for the piano (37.12%) as their pitch source, followed closely by the pitch pipe (33.33%).