The article presents an analysis of the factorial structure and measurement invariance of the Innovative Behavior Questionnaire, developed by Scott and Bruce. Although the instrument is widely used to capture individuals' innovative behavior, very little evidence concerning its psychometric properties is available. A time-lagged study among 382 employees was conducted to check the factorial structure of the questionnaire, using confirmatory factor analysis, as well as its measurement invariance across gender and time. One-factor structure (with correlated error terms of first three items) and strict invariance across time and across gender of the Innovative Behavior Questionnaire were demonstrated. As such, the measure can be used as a reliable tool for capturing individuals' innovative behavior by self-report.