The plant taxonomy textbooks that are generally used in colleges need to be complemented with a DNA-based molecular review. In general, plant taxonomy textbooks devote more space to morphology than to molecular-level characteristics. The research reported here used the results of DNA-based research to develop a molecular plant taxonomy textbook. The purpose of this research was to develop a suitable plant taxonomy textbook that was valid, practical, and effective. The textbook is useful to provide students insight into plant taxonomy from a molecular perspective. To achieve that goal we used the Plomp development method, which consists of five stages: (1) the initial assessment phase, (2) the design phase, (3) the realization /construction phase, (4) the test, evaluation and revision phase, and (5) the implementation phase. The quality of the plant taxonomy textbook based on a molecular approach was assessed using following criteria: validity, practicability, and effectiveness. Further testing of the quality of the textbook and supporting devices was conducted with biology students taking a Higher Plant Botany course. Analyses of these data showed that the textbook met the validity, practicality, and effectiveness standards.