We investigated class clustering patterns of four behaviors--physical activity, fruit and vegetable (F&V) intake, smoking, and alcohol use--in a faith-based African American cohort. Guided by socio-ecological models, we also examined the psychosocial and neighborhood social environmental factors associated with the clustering patterns. Participants were 1,467 African American adults recruited from a mega church in the metropolitan Houston, TX, in 2008-2009. They completed a survey and health assessment. Latent class analysis and multinomial regression analysis were conducted. Results supported a three-class model: Class 1 was characterized by low physical activity, low F&V intake, and low substance use (smoking and alcohol use). Class 2 was characterized by high physical activity, low F&V intake, and mild drinking. Class 3 seemed to be the healthiest group, characterized by high physical activity, moderate-to-high F&V intake, and low substance use. The probabilities of being included in Classes 1, 2, and 3 were 0.33, 0.48, and 0.19, respectively. Participants in Class 1 (vs. Class 3) reported lower physical activity norm (p < 0.001) and higher smoking norm (p = 0.002) and lower neighborhood social cohesion (p = 0.031). Participants in Class 2 (vs. Class 3) reported higher cancer risk perception (p < 0.001), lower F&V norm (p = 0.022), lower physical activity norm (p < 0.001), higher smoking norm (p < 0.001), and lower social cohesion (p = 0.047). As health behaviors are clustered together, future interventions for African Americans may consider targeting multiple health behaviors instead of targeting a single health behavior. Interventions addressing social norm and neighborhood social cohesion may enhance multiple health behaviors engagement in this population.