When athletes fail to perform at an expected level during an important moment, it is implied the athletes have experienced "choking" (sudden decline in performance) under pressure.". Researchers have reported that persistent left-hemispheric activation patterns occur when an athlete experiences considerable performance deteriorations under pressure. Researchers have also observed differences in brain activation patterns between left- and right-handed people on a variety of physical and cognitive tests, with the left-hemispheric activation more pronounced in right-handed participants. Purpose: The purpose of this study was to investigate whether athletes' handedness may be linked to choking susceptibility (i.e., likelihood to experience performance decline under pressure). Method: Twenty right-handed and 13 left-handed experienced Australian football players completed 15 shot attempts, in both a low-pressure and a high-pressure condition. Both groups displayed equal state anxiety increases due to the pressure manipulation, indicating similar increases in anxiety in both handedness groups. Results: Differences were indicated in performance between the left- and right-handed groups during the high-pressure condition, with the left-handed group maintaining, and the right-handed participants declining, performance. Conclusion: Future electroencephalogram (EEG) research investigating this link may clarify the effect between handedness and choking.