Use of captioned video in classrooms has gained considerable attention in the second and foreign language learning. However, the effect of application of captioned video embedded with glosses on incidental vocabulary enhancement has not been explored. This study aims to examine the effect of video captions with glosses on EFL students' incidental business vocabulary acquisition; 50 students from a college of management served as participants. A pretest was adopted to ensure participants lacked familiarity with the target vocabulary. All participants watched three video clips presented in three modes (noncaptioned, captioned, and caption-gloss modes). After each mode session, all participants took an immediate posttest and a 3-week-delayed posttest. Following the final posttest, the participants completed a questionnaire. The findings revealed that the caption-gloss mode significantly outperformed the other 2 modes in both the immediate and 3-week-delayed posttests. Retention of the target business words did not significantly decline at the 3-week-delayed posttest. Therefore, glosses in the captioned video improved the participants' short- and long-term incidental business vocabulary retention. The participants also provided positive feedback regarding the efficacy of the caption-gloss mode for incidental business word acquisition. Pedagogical implications regarding use of captioned video with glosses for incidental professional vocabulary acquisition are discussed.