ERIC Number: EJ1214830
Record Type: Journal
Publication Date: 2019
Pages: 15
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-1470-8175
EISSN: N/A
Available Date: N/A
A Research-Inspired Biochemistry Laboratory Module--Combining Expression, Purification, Crystallization, Structure-Solving, and Characterization of a Flavodoxin-Like Protein
Hammerstad, Marta; Røhr, Åsmund K.; Hersleth, Hans-Petter
Biochemistry and Molecular Biology Education, v47 n3 p318-332 May-Jun 2019
Many laboratory courses consist of short and seemingly unconnected individual laboratory exercises. To increase the course consistency, relevance, and student engagement, we have developed a research-inspired and project-based module, "From Gene to Structure and Function". This 2.5-week full-day biochemistry and structural biology module covers protein expression, purification, structure solving, and characterization. The module is centered around the flavodoxin-like protein NrdI, involved in the activation of the bacterial ribonucleotide reductase enzyme system. Through an in-depth focus on one specific protein, the students will learn the basic laboratory skills needed in order to generate a broader knowledge and breadth within the field. With respect to generic skills, the students report their findings as a scientific article, with the aim to learn to present concise research results and write scientific papers. The current research-inspired project has the potential of being further developed into a more discovery-driven project and extended to include other molecular biological techniques or biochemical/biophysical characterizations. In student evaluations, this research-inspired laboratory course has received very high ratings and been highly appreciated, where the students have gained research experience for more independent future work in the laboratory.
Descriptors: Biochemistry, Science Laboratories, Laboratory Training, Science Process Skills, Molecular Biology, Molecular Structure, Genetics, Scientific Research, Student Projects, Active Learning
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Publication Type: Journal Articles; Reports - Descriptive
Education Level: N/A
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A
Author Affiliations: N/A