The purpose of this study was to develop and validate a scale aiming to assess pre-service teachers' attitudes towards 3D printing for teaching and learning processes. The data were collected from 250 pre-service teachers in different departments at a public state university. The validity of the scale was tested with exploratory factor analysis (EFA) and confirmatory factor analysis (CFA). As a result of EFA, the scale consisted of three factors; perceived ease of use, perceived usefulness, and intention to use, with 18 items in 5 point Likert scale. The fit values from the CFA confirmed the three-factor structure of the scale. The correlations among the factors were positive, and collinearity among the factors was not evident. The scale and factors demonstrated a satisfactory level of internal reliability, ranging from 0.68 to 0.94. The findings of this study showed that the scale was valid and reliable, and could be used by researchers interested in technology integration in teaching and learning environments.