India is a growing ICT country in the world. In the most recent research findings ICT provides us several ways of learning and it also affects teachers by providing alternative teaching styles. With the use of the latest technology, teachers can grasp the significance of technology. To use it efficiently and creatively, the teacher should have right and positive attitude. By keeping this thing in view, the concerned study describing the attitude of Indian teachers towards ICT in education in relation to their affiliation with the University is conducted. More than three hundred teachers from different faculties of six universities located in two states of India have participated in the academic year 2015. The statistical test like Z-test and T-test at 5% confidence level proves that University affiliation did not affect the teachers' attitude towards ICT in higher education.