The rationalization of this research was an exploration and discrimination about humanizing research culture system through Quality Assurance Practices (QAP) in the Universities in Pakistan pertaining to the views of students, teachers and Directors of Quality Enhancement Cells (QECs). This study had a mixed method design such as quantitative and qualitative. The study was conducted on a sample of 28 universities (public and private sector) of Pakistan by using multi-stage (random, convenient and purposive) sampling technique. Questionnaires and semi structured interviews were used to congregate information from the determined samples. The data was analyzed by using inferential statistics and SPSS version 16. The study reflected that participants faced a lot of problems without research culture system in higher education. On the basis of data analysis, key findings of the study were derived such as seminars are conducted/arranged, workshops are arranged to trained the man powers, follow up systems is done after training, research projects are completed, allowed teachers to participate in seminars, collaboration with other departments is available and publication of articles are being carried out among universities to enhance the research culture system. Lack of physical facilities, no training for faculty, provision of funds, research journals, communication system and feedback system were major problems faced by the Quality Assurance Practices with reference to research culture in the universities. Following suggestions can be accelerated to humanizing research culture system similar to provision of sufficient resources, addition of latest software, provision of modern professional development skills for academic staff, feedback system and provision of latest research journals.