This study aims to evaluate the implementations of cooperative learning method in teaching limit and derivatives and the opinions of prospective teachers and instructors about these implementations. The research is designed as a case study and conducted with 28 prospective teachers (20 female, 8 male) who were attending the course of calculus in the department of science and technology teaching. The data obtained by interviews and observations were analyzed via content analysis. According to results, it was found out that the implementations of cooperative learning method created a positive effect on prospective teachers and made them learn the subjects more easily. On the basis of the results mentioned above, it can be recommended to use the cooperative learning method in teaching the subjects of limit and derivatives in teacher education. This study focused on teaching the subjects of limit and derivative; however similar studies can be carried out with different mathematics topics.