This research aimed to examine the effectiveness of a Generative Learning-based biology module to improve the analytical thinking skills of the students with high and low reading motivation. The method used was a 2x2 factorial design. The participants were 250 students of the Class XI SMA Kota Kotabumi. The researcher used an intact group technique to determine the sample. The sample was divided into two groups, namely the control group using conventional modules and the experimental group using Generative Learning-based biology modules, and each sample groups were divided into two groups of high reading motivation (HRM) and low reading motivation (LRM). The instrument used to obtain the data of analysis capability was an essay test, the indicators based on Facione's analytical thinking skills. The instrument of reading motivation used the Motivations for Reading Questionnaire (MRQ). The results of the data analysis showed the effectiveness of the Generative Learning-based biology module implementation on the students' analytical thinking skills. There was not only an influence of reading motivation through students' analytical thinking skills but also interaction between the module and students' reading motivation towards their analytical thinking skills.