The aim of a pillar research was to find a change in a content of a physical and sport education through a targeted application of a physical program for three months, with a focus on a dynamic function of female students' spine. The monitored group consisted of the female students of an adolescent age (age 16.32 ± 0.42 years, height = 169.56 ± 6.33 cm, weight = 57.32 ± 8.76 kg) of the town, Banská Bystrica who attended the Secondary Health School. From the point of view of data acquisition methods, standardized tests for a physical education and a medical practice were applied. The results in the dynamic spine function of the female students were recorded in an initial assessment during a supervisory period in the most cases of deviations from the standard, in the noncompliance standard in all tests, which means that weaknesses in the dynamic spine function were recorded among all probands. During a final assessment in the supervisory period were recorded only minimal changes, statistically insignificant (p > 0.05) in all tests, except for right and left lateroflexion (p < 0.05, Z = -2.1181, r = 0.43), while in an experimental period after application of the physical program we recorded significant changes with a high effect of an effect size, indicating that the results were not influenced by statistical data (p < 0.01, Z = -3.0594, r = 0.62) in all five assessed areas of the dynamic spine function. This project was supported by VEGA 1/0242/17, "Physical activity as prevention of functional disorders related to the musculoskeletal system of secondary school students."