This study focused on the perception of medicine as a calling and its associations with medical training satisfaction, career certainty, career satisfaction, career commitment, and well-being among 176 Israeli medical students. Participants replied to an online questionnaire including the Brief Calling Scale (Duffy and Sedlacek in Career Dev Q 59:27-41, 2010) and measures related to medical training, a medical career, and well-being. Calling was positively associated with all outcome measures, and gender moderated these associations, which were stronger for female medical students in comparison with male medical students. The importance of taking these gender differences regarding calling under consideration for career counseling is discussed.