An evidence-based research mentor training (RMT) curricular series has been shown to improve the knowledge and skills of research mentors across disciplines and career stages. A train-the-trainer model was used in the context of several targeted approaches aimed at sustainability to support national dissemination of RMT and expand the network of facilitators prepared to implement the curricula. These infrastructure elements included (1) an expansion initiative to increase the number of trained facilitators able to deliver train-the-trainer workshops nationwide; (2) adaptation of RMT curricula for multiple audiences and career stages to increase accessibility; (3) implementation resources to support facilitators and help them overcome implementation barriers; and (4) standardized evaluation of training. This approach to dissemination and implementation has resulted in the preparation of nearly 600 trained facilitators, a large percentage of whom have implemented mentor training for more than 4000 graduate student, junior faculty, and senior faculty mentors. Implications for and challenges to building and sustaining the national dissemination of RMT are discussed.