In the present study we aimed to examine the relationship between competitive anxiety, autonomic nervous system activity represented by Heart Rate Variability (HRV) and respiration, and performance in novice archers. We also interested in whether neuroticism may be a factor with a potential to moderate the link among anxiety, autonomic activity and archers' performance. Methods: The sample included 39 novice archers ranging in age from 18 to 27. Participants first completed to anxiety and neuroticism measures. Then, participants' HRV and respiration activity was measured for 4 minutes. Afterwards, participants shot 10 arrows from 18 m to an 80 cm diameter target while their HRV and respiration was measured. Shooting accuracy was determined in terms of radial distance (error) from the center of the target. To determine an overall performance score, the median value of the 10 arrows was calculated for each participant. Spearman correlation analyses together with linear regression were used to analyze obtained data set. Results: Overall, results indicated an association between respiration and anxiety. Moreover, results provided preliminary evidence that neuroticism may be a factor having potential to moderate the relationship between anxiety and autonomic activity before and during an archery competition. Discussion: Results of the present study suggested that determination of archers' performance should be based upon both physiological and psychological factors.