Nigerian university teachers' level of computer skills acquisition and programme mastery was analysed in this study. 367 university teachers with mean age of 41.21 and Standard deviation of 9.56 drawn from 2 universities in Ogun-state, Nigeria were used as the sample for the study. The Computer Skills Acquisition and Progarmme Mastery Questionnaire was used for data collection. The data collected was analysed at 0.05 level of significance using the t-test and Analysis of Variance statistical tools. The results indicated that gender had a great influence on the level of computer skills acquisition and programme mastery of university teachers. Meanwhile, as the computer skills acquisition level of the university teachers was certain to specific academic qualification, their level of computer programme mastery was not dictated by their level of academic qualifications. It was among other things recommended that in all universities, students of Faculty of Education course should be made to undergo computer appreciation course. The university teachers are taken through computer training in order to improve their level of computer literacy, while the universities management should endeavour to make personal computers available to every university teacher in order to facilitate effective teaching and learning.