MATLAB is a powerful package for numerical computation. MATLAB contains a rich pool of mathematical functions and provides flexible plotting functions for illustrating mathematical solutions. The course of calculus-based business mathematics consists of two major topics: 1) derivative and its applications in business; and 2) integration and its applications in business. Both topics involve various mathematical functions that may scare many business students. Fortunately, MATLAB provides an easy solution to handling the derivative and integration of various mathematical functions. With the help of MATLAB, all elementary formulas for derivative and integration in the textbooks of calculus-based business mathematics become less important to those students who have difficulty in learning and using mathematical formulas to find solutions to quantitative business problems. With the wide availability of various business data bases including the MATLAB package in the Samuel S. Bergami, Jr. Learning Center for Finance and Technology at the University of New Haven, in this paper, we illustrate how to use MATLAB to help students? learning of calculus-based business mathematics and apply the calculus principles to find solutions to real quantitative business problems. Our experience shows that MATLAB greatly saves students? efforts in learning difficult and pure calculus formulas and it is easy for business students to acquire the basic internal functions in using MATLAB.