The purpose of the present study was to examine the psychometric properties of the Chinese version of the Acceptance and Action Questionnaire-II (AAQ-II) across two samples of Chinese college students (n = 183 and n = 366) and a sample of elite Chinese athletes (n = 330). Exploratory and confirmatory factor analyses supported the existence of a unidimensional AAQ-II. Adequate internal consistency reliability, test-retest reliability (one-month interval), factorial validity, and nomological validity with mindfulness, well-being, positive and negative affect/mood for both students and athletic samples were demonstrated. The AAQ-II also showed incremental validity in college students in explaining variances of well-being, positive and negative affect, anxiety, and depression, beyond the mindfulness measure. Most important, factorial invariance of the AAQ-II was demonstrated across male and female college students as well as across male and female athletes. Partial factorial invariance was also demonstrated across adolescent and adult athletes. Overall, results of this study suggest that the Chinese version of the AAQ-II may be a useful self-report measure of experiential avoidance in Chinese college students and elite Chinese athletes.