The Human Immunodeficiency Virus (HIV) prevents the immune system from fighting infections effectively. One in 48 Black women will acquire HIV throughout their life if current trends continue. Black women have the highest HIV infection rates compared to White and Hispanic women. In 2019, 55% of women with HIV in the United States were Black. There is a gap in public health research on African American women's perceptions of pre-exposure prophylaxis (PrEP) and HIV. The purpose of this qualitative study was to explore PrEP uptake among heterosexual, cisgender Black women attending a Historically Black College and University (HBCU) in the Northeastern United States. The purposive sample included 22 Black women currently enrolled at an HBCU assigned female at birth aged 18-49. Five virtual 60-minute focus groups were conducted over two months and transcribed verbatim. The study yielded four major themes: (i) knowledge and awareness of PrEP, (ii) perceptions of PrEP, (iii) facilitators of PrEP uptake, and (iv) barriers to PrEP uptake. In conclusion, there was a gap in overall sexual reproductive health knowledge and PrEP among Black women attending HBCUs. Future public health efforts should utilize an individualized, culturally appropriate framework for PrEP delivery and uptake for Black women to minimize the risk of acquiring HIV. Additionally, HBCUs need to explore the unique perspectives, attitudes, and knowledge surrounding PrEP uptake among Black women attending college. There is also a need for increased PrEP uptake by Black women attending HBCUs, which is imperative for national HIV prevention efforts. [The dissertation citations contained here are published with the permission of ProQuest LLC. Further reproduction is prohibited without permission. Copies of dissertations may be obtained by Telephone (800) 1-800-521-0600. Web page: http://www.proquest.com.bibliotheek.ehb.be/en-US/products/dissertations/individuals.shtml.]