Research suggests that physical therapists lack knowledge, awareness, and clinical preparedness to engage with LGBT+ populations in clinical settings and that LGBT+ patients report difficulty engaging in physical therapy and fear of discrimination. The purpose of this study was to develop, deliver, and evaluate an online LGBT+ culturally competency training (CCT) program for physical therapists. To address the purpose, physical therapists in current clinical practice completed surveys assessing LGBT+ clinical preparedness, attitudinal awareness, and basic knowledge before and after engaging in an LGBT+ clinical competency training. An initial sample (n=115) completed the pretest, with only 30 completing the course and participating in the study. Participants' scores on all 3 subscales and total of the Lesbian, Gay, Bisexual, Transgender Development of Clinical Skills Scale (LGBT-DOCSS) were significantly higher at post-test, with the greatest increase in clinical preparedness, t(29) =7.15, p<0.001, and a large effect size (d=1.30). These findings suggest that participating in this course increase PT's LGBT+ cultural competency. The majority of participants (96%) agreed that LGBT+ cultural competency is essential to PT curricula, should be CAPTE mandated in PT programs, and clinical practice guidelines should be published. Post-course ratings of course content, delivery, and format were all very positive. The course was successful in raising cultural competency with a large effect for clinical preparedness and was rated positively by participants. [The dissertation citations contained here are published with the permission of ProQuest LLC. Further reproduction is prohibited without permission. Copies of dissertations may be obtained by Telephone (800) 1-800-521-0600. Web page: http://www.proquest.com.bibliotheek.ehb.be/en-US/products/dissertations/individuals.shtml.]