This study investigated the effect of the isolation during the lockdown period on psychological distress and well-being in a sample of professors (N=150), students (N=150) and technical administrative staff (N=150) from 3 Italian Universities, in the region of Tuscany. We administered a self-reported online questionnaire to collect socio-demographic information, investigate issues in work and study life, and assess the level of perceived stress and psychological well-being. The results of this study indicate that under conditions of isolation perceived stress levels are increased, and this is related to the experience of an unpleasant house environment. This is also significantly predicted by the occurrence of issues with online lectures and administrative activities for university professors or by having issues with managerial support for technical administrative staff. On the other hand, for university students, a good relationship with other students is of great importance to maintain a high level of mental well-being and being male is a protective factor. Evidently, aspects related to the deprivation of opportunities for peer relationships are experienced as particularly problematic. [For the full proceedings, see ED621108.]