Science is a systematic study of natural phenomenon. The natural phenomenon is perceived and understood by an individual based on his/her experience and cultural context. The term misconception is complex in nature and has been conceptualize as alternative conceptions, preconceptions, alternative ideas, conceptual stumbling blocks, erroneous concepts, alternative framework in various contexts by researchers at different level of schooling and geographical locations. Identification of misconception is a challenging task due to various factors such as cultural context, individual differences, teaching-learning processes, students personal experience etc. In the present context of multi and trans-disciplinary subjects, the identification of misconceptions has become essential and gain importance as they are contributing new knowledge and applications in Science and Technology. Hence, in the present paper researchers had made an attempt to analyze the intricacies in identification of misconceptions in biology. The paper heavily relies on secondary source of data. In the present paper researcher has thoroughly reviewed and studied the identifications of misconceptions in the subject biology mixed approaches along with tools, techniques and strategies.