In spite of the trend toward online learning in higher education in Australia, few research examined university students' online learning attitude changes and the factors which could predict their attitude changes. The paper aimed to investigate 120 Australian university students' attitudes toward online learning in a blended course. The factors, such as prior attitudes, prior experiences, and motivation, were examined with the participants' online learning attitudes by the end of the course. Two phases in the study included the pre- and post-test and course participation (developed from the data of the students' weekly reports and online forum transcripts) in Phase One and interviews in Phase Two. It was found that the students became more positive toward online learning by the end of the course. The predictors for their online learning attitudes by the end of the course were their prior online learning attitudes and motivation in learning.