The present study aims to investigate attitudes and readiness of Information and Communication Technologies pre-service teachers toward web-based learning. In the study group of the research are 165 pre-service teachers. The study was conducted in the survey model. As data collection tools, 'Web-based Teaching Attitude Scale' and 'Readiness and Expectation Scale for eLearning Process' were utilized. In analysis of the collected data, descriptive statistics, t-test, and ANOVA analysis were conducted. As a result of the research, it was found that attitude levels of pre-service teachers were lower than median; and their readiness levels were above the median. Moreover, in terms of gender, there was no significant difference between attitude and readiness scores. While there was no significant difference among attitude scores in terms of number of years in education, there was significant difference in readiness scores. [This paper was presented at the International Conference on New Horizons in Education, INTE 2014.]