Research has indicated the importance of evaluating the experiences related to developing e-portfolios (electronic portfolio) to contribute to the overall excellence in teaching and learning. Researchers (instructors) of the present study used an exit e-portfolios, a questionnaire and follow up interviews to describe development and evaluation of e-portfolios with teacher education graduates in their final capstone experience at a major UAE (United Arab Emirates) national university. There is not any such research study conducted in the UAE and there are few studies conducted, in the topic, internationally. This study described the process of developing an e-portfolio and examined its validity and participants' perceptions to assess teaching performance in the UAE. E-portfolios and surveys of 67 pre-service teachers were included in the analysis and findings. A questionnaire was developed by the authors to examine common perceptions. Analyses of quantitative and qualitative data were presented. A major result indicated that the use of e-portfolio is considered as a pragmatic vehicle to assess pre-service teachers' performance and evaluate teacher education programs. (Contains 2 tables.)