This document reports on an assessment of the Jacksonville State University's Houston Cole Library collection that employed a variety of methodologies and tools, including list-checking, direct collection examination, shelflist measurement and analysis, WLN (Washington Library Network) conspectus sheets, analysis of OCLC/AMIGOS Collection Analysis Service statistics, and compilation of percentage of growth statistics. It is a base for planning acquisitions activity and collection treatment, measuring whether the library conforms to its collection development policy, and determining whether program or accreditation requirements are being addressed. Reviews are presented of holdings in 34 subject areas: agriculture, Alabama Gallery, American literature, art, audiovisual, auxiliary sciences of history, biology, chemistry, commerce and business administration, communication, criminal justice, drama, education, English literature, family and consumer science, general works, geography and anthropology, geology, government documents, history, languages and related literatures, law, library science and bibliography, mathematical and information sciences, military science, music, nursing, philosophy and religion, physics and engineering, political science and public administration, psychology, sociology and social work, technology, and Western European literatures. Each review contains statistics on monographic, serial, and nonprint media holdings; a description of support from collections in related subjects areas; and summaries of strengths, weaknesses, and recommendations. (KRN)