The Sensory Integration Infant Routines Questionnaire (SIIRQ) is designed to identify sensory integration difficulties through observation of participation in infant co-occupations. Identifying sensory integration and participation issues at an early age is of utmost importance given that these difficulties can affect many areas in the child's life and consequently, the family's dynamics and routines. This study examined the construct validity of the SIIRQ using hypothesis testing to differentiate between typically developing children (n = 1219) and children with identified sensory integration issues (n = 75). The difference between the two groups was statistically significant in the various subscales, with the exception of the Diaper Changing/Sphincter Control subscale. Results support using the SIIRQ to identify sensory integration difficulties by questioning caregivers about daily activities inherent to the routine of the child and family. We hope that the SIIRQ can be used by health and education professionals who deal with children on a daily basis to enable referral for a comprehensive and detailed evaluation of sensory integration and participation challenges.