Background: The voice range profile (VRP) is an acoustic measurement in vocal function voice assessment. While several factors influencing VRP outcomes are known, the impact of body posture during VRP recordings remains unexplored. Aims: To investigate the effects of standing and sitting posture on VRP performance in vocally healthy individuals. Methods and Procedures: Thirty vocally healthy and untrained participants were randomised into two groups. Group 1 performed VRP measurements first in a sitting position, followed by standing. Group 2 completed the tasks in reverse order. VRP parameters were compared between sitting and standing positions, and training effects between the first and second measurements were analysed. Outcomes and Results: No significant differences were found in any VRP parameter between sitting and standing positions (all p values > 0.05), with nearly all effect sizes being very small or small. Furthermore, no training effect was observed among the two trials in each group (all p values > 0.05, ICC > 0.75, very small or small effect sizes). Conclusions and Implications: Posture (sitting vs. standing) did not significantly influence VRP performance in the first instance when considering people without voice disorders. This might suggest flexibility in patient positioning during VRP assessments, potentially simplifying clinical protocols without compromising data integrity.