This study aims to determine the e-learning readiness of Turkish family physicians and to obtain the views of program instructors to design the e-learning system. An online questionnaire was applied, and 1172 family physicians answered, and a semi-structured interview was done with the program educators. Infrastructure and equipment readiness was 77.64%, and there was a need for quantitative and qualitative improvements. Online learning readiness was 75.13%, and efforts were needed to develop education and online culture. Information technology readiness was 74.47%, and there was a need to develop information technology knowledge. Attitude readiness was 64.22%, and it was insufficient; it was understood that behaviour and desire need to be developed more to improve. Considering demographic characteristics, age was the strongest and most significant predictor of e-learning readiness. In the interviews with the program trainers, e-learning training, development of human resources capacity, increasing enthusiasm for technology, use of asynchronous course format, and development of online culture were indicated for improving an e-learning system for trainers. It was concluded that information technology readiness moderately affected online learning and attitude readiness but slightly affected equipment and infrastructure readiness. As a result, it showed that 72.80% of Turkish family physicians were ready for e-learning and needed several improvements for sufficient e-learning readiness.