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ERIC Number: EJ1490578
Record Type: Journal
Publication Date: 2025-Nov
Pages: 7
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-1470-8175
EISSN: EISSN-1539-3429
Available Date: 2025-08-13
ABCC11 Earwax Trait and Genotype Are Suitable Tools for Introductory Labs to Learn Genetics and Molecular Techniques
Tohru Ohta1; Rie Takai2; Akiko Yoshida3; Durga Paudel1; Sarita Giri1; Takao Kitagawa1; Toshiya Arakawa2; Yasuhiro Kuramitsu1; Tomoharu Tokutomi4
Biochemistry and Molecular Biology Education, v53 n6 p603-609 2025
Professional experiments in genetic research usually start in a class at university. However, interest in genetic research techniques from an early age is essential. We have continuously performed a short genetic experimental course for high school students using a simple molecular experiment and computer-based learning for Mendelian inheritance. We utilized the phenotype-genotype association of ABCC11 polymorphism (rs17822931), in which the A allele at rs17822931 of ABCC11 is the recessive genotype for the dry earwax phenotype. Conversely, the G allele is the dominant genotype for the wet earwax phenotype. The phenotype is primarily determined by a single-nucleotide polymorphism (SNP), rs17822931, with the A or G allele, which has global prevalence with few exceptions. The A or G allele is easily typed using the DdeI restriction enzyme. The students experienced molecular techniques and created computer-equipped pedigree charts using the software "f-tree." The earwax trait is an excellent tool for genetic education to understand Mendelian inheritance, genotype-phenotype association, PCR cloning, and restriction enzyme digestion, and it is suitable for discussing the historical and geographical migration of the ancient Mongolian people. After the short course, a survey showed that 81% of students were satisfied, including 71% of very satisfied students.
Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www-wiley-com.bibliotheek.ehb.be/en-us
Publication Type: Journal Articles; Reports - Research
Education Level: Secondary Education; High Schools
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A
Author Affiliations: 1Advanced Research Promotion Center, Health Sciences University of Hokkaido, Tobetsu, Japan; 2Division of Biochemistry Department of Oral Biology, Health Sciences University of Hokkaido, Tobetsu, Japan; 3Department of Clinical Genetics, School of Medicine, Iwate Medical University, Morioka, Japan; 4Department of Pediatrics, Kawasaki Medical School, Kurashiki, Japan