Describes a science activity designed for undergraduate biochemistry classes. Provides background, a list of materials, experimental procedures, and the results of this experiment. (CW)

Presents a practical exercise that enables students to measure flux control coefficients in a metabolic pathway in vitro, to verify the summation property, and to compare the differences in the distribution of these coefficients in different steady states. Discusses background, materials, methods, and results of the activity. (CW)

Describes the construction of an inexpensive apparatus for separating proteins by polyacrylamide gel electrolysis in the presence of sodium dodecyl sulphate. (TW)

Suggests a new experiment to study the genetic material of eukaryotes. It is based on the rapid growth and interest of the research on chromatin. (TW)

Describes an experiment to identify lactose and estimate the concentration of lactose in a sample of milk. Gives a background of the investigation. Details the experimental method, results and calculations. Discusses the implications of the experiment to students. Suggests further experiments using the same technique used in…

Offers a series of experiments which introduce students to the general principles of enzymology. The experiment demonstrates several basic enzyme properties and the chromatographic exercises provide an analysis of each enzymatic activity. Questions are also presented for extending discussion on the activities. (ML)

Describes a computer simulation program which helps students learn the main biochemical tests and profiles for identifying medically important bacteria. Also discusses the advantages and applications of this type of approach. (ML)

Background information and procedures are provided for a demonstration of intramolecular versus intermolecular hydrogen bonding. The demonstration is based on structural changes in bilirubin molecules which lead to changes in physical properties. A list of further investigations to try is included. (JN)

Provides sources of nonanimal biochemical materials (which are relatively inexpensive, readily available, and require no special storage) suitable for use in biochemistry experiments. They are presented under these headings: (1) enzymes and other proteins; (2) carbohydrates; (3) lipids; (4) nucleic acids; and (5) metabolism. (JN)

Describes a set of experiments (for senior-level biochemistry students) which permit evaluation and estimation of rate and equilibrium constants involving an intermediate in the alpha-chymotrypsin mediated hydrolysis of ortho-hydroxy-alpha-toluenesulfonic acid (I). The only equipment required for the experiments is a well-thermostated double beam…

Discusses structural and chemical features of muscle tissue. Emphasizes chemical and physical changes which occur during processing and storage and how these changes affect the eating quality of meat and fish. (JN)

Understanding chemical reactivity, chemical catalysis, and the chemistry of life processes are cited as key areas for government research funding in a National Academy of Sciences/National Research Council committee briefing to White House Office of Science and Technology Policy. Fundamental questions to be answered in each area are outlined and…

Describes some recent advances in enzyme structure and action, including a description of enzyme-substrate interaction. Discusses the methods for determination of amino acid sequences in proteins; the actions of chymotrypsin, trypsin, and elastase; and details of the enzyme-substrate complex derived from kinetic studies and x-ray diffraction…

Examines recent studies of the elimination-addition mechanism of acyl group transfer, in which an acid function moves from one acceptor to another. Presents diagnostic evidence for this mechanism and discusses acyl group transfers in metabolism. (MLH)

Describes a biochemistry experiment that illustrates the methodology of affinity chromatography by attaching avidin, a glycoprotein in egg white, to a Sepharose matrix in order to bind biotin-containing proteins. (MLH)