The choice of cortisol sampling times in early childhood studies varies widely. Given that recommendations on sampling protocols are largely based on adults, the present study aimed to broaden current knowledge by examining how reliably cortisol measures obtained at different daytimes would reveal between-individual differences in toddlers'…

Reward is thought to attenuate forgetting through the automatic effect of dopamine on hippocampal memory traces. Here we report a conceptual replication of previous results where we did not observe this effect of reward. Participants encoded eight lists of pictures and recalled picture content immediately or the next day. They were informed that…

Dynamic biochemical processes are often difficult for students to grasp in the classroom using static images or models, or even through watching simulation videos. This is particularly true for protein-folding and enzyme catalysis, two key biochemistry concepts. The author has developed and tested two 3D, dynamic, and interactive classroom models,…

Although biochemistry is fascinating to undergraduates regardless of their major, the complexity involved requires a great deal of effort and leads many to perceive it to be an intense, facts-based course limiting personal creativity. This activity takes the fundamental knowledge associated with inducible promoters and combines it with the…

Second-semester biochemistry students at two primarily undergraduate institutions, the California State Polytechnic University, Pomona, and the State University of New York College at Cortland, worked on a collaborative, semester-long project that resulted in the production of bioinfographics focused on metabolic pathways. Students were divided…

The field of biosensors is a burgeoning area of research and employs a large number of chemistry graduates. The impact of strip tests in detecting coronavirus was palpable during the recent COVID-19 pandemic and will further drive the biosensor industry. Despite their common usage, a coherent introduction to the basics of sensing remains missing…

Exposure to real-life applications of quantitative analysis techniques based on two gas chromatography (GC) experiments was provided to a large cohort of sophomore-level students. In one experiment, students assessed blood alcohol content by internal standardization using a homemade headspace system similar to methods implemented in forensic…

Enzyme-linked immunosorbent assays (ELISAs) are used widely in biotechnology, pharmaceutical, and clinical medicine labs. At the same time, they appear to be underrepresented in chemistry and biochemistry curricula, even though their sensitivity, selectivity, and ease of use would argue for their widespread use. We describe here an online ELISA…

The Hill equation, which models the cooperative ligand-receptor binding equilibrium, turns out to be useful in modeling the progression of infectious disease outbreaks such as CoViD-19. The equation fits well the data for total and daily case numbers, allows tentative predictions for the half-point and end point of the epidemic, and presents a…

The use of the thermodynamic formalism in the investigation of biochemical reactions constitutes one of the key analysis in bioenergetics, and the first step in such analysis is the selection of the adequate reference state. For biochemistry majors, thermodynamic analysis based on the chemical reference state is used in Physical Chemistry courses,…

Previously most studies indicated that high intensity training is a powerful stimulus to acute increases in blood testosterone hormone levels in males. The testosterone responses to exercise in females are controversial; while some studies have shown increases, others have suggested no change. The aim of this study was to determine alterations in…

Biochemistry is about structure and function, but it is also about data and this is where computers come in. From my time as a graduate student and post doc, whenever I encountered data I thought, "I can work this up by hand, but I think a computer could do a better job." Since that time, I have been working at the interface of…

The following story tells the details of the ketone body metabolism, focusing on the needs of the brain in prolonged fasting conditions. This literary composition highlights the use of ketone bodies as an important metabolic adaptation that avoids excessive protein degradation and allows for greater survival. The liver produces ketone bodies that…

In this article, we describe an in-house polymerase chain reaction-sequence specific priming (PCR-SSP) assay designed for undergraduate medical students as part of the experimental pathogen biology and immunology (EPBI) course. It screens human leukocyte antigen (HLA)-DR2 allotype from genomic DNA samples using a rapid and single-tube PCR…

The authentic teaching and learning approach introduces real-world scenarios into the classroom to better engage Generation Z students. Considering this, we introduced an authentic learning practical exercise (breakfast meal and glycemic variation) to undergraduate biology students at the University of La Reunion (France). Here, students were…