This study focused on the investigation of the spiritual intelligence of prospective teachers with respect to their gender, locality, and stream. A descriptive survey method was used along with the sample of 105 prospective teachers through multistage sampling techniques. A standardized Sixfold Spiritual Intelligence Scale was used for data collection. Data were analyzed through SPSS software using statistical analysis, percentage analysis, mean, SD, and t-test. The study revealed that prospective teachers had a high level of spiritual intelligence in relation to their gender, locality, and stream, and there is no statistically significant difference in spiritual intelligence of prospective teachers between male and female, urban and rural, and arts and science. The investigator suggested that, for the development of spiritual intelligence among the students, the educational institutions, starting from pre-primary to higher education, should organize various seminars, conferences, workshops, yoga, and meditation programs. Besides this, parents should emphasize it and create such a conducive environment for the development of spiritual intelligence of the whole family at their home.