Raman scattering spectroscopies are important analytical tools in modern chemistry. Here, we describe the construction of the unamplified Raman scattering analysis (URSA) instrument: an inexpensive, open-table Raman spectrometer that uses a 532 nm diode-pumped laser as a light source. Upon application of this spectrometer to DNA extracted from…

This exercise encompassed the construction and use of a microcontroller-based laser refractometer. The instrument, which does not require a high skill level to build, primarily consisted of a line laser, optical sensor, several 3-D printed parts and printed circuit boards, and a microcontroller development board. Construction and testing of the…

A low-cost time-resolved emission spectrometer optimized for ruby emission is presented. The use of a Class II diode laser module as the excitation source reduces costs and hazards. The design presented here can facilitate the inclusion of time-resolved emission spectroscopy with laser excitation sources in the undergraduate laboratory curriculum.…

We have developed a laboratory project in which students prepare liposomes, expose them to hyperosmotic and hypoosmotic solutions, and follow the resulting shrinking and swelling (respectively) with laser light scattering. Each light intensity transient can be fit to an exponential decline or rise, with the decay constant (k) and the amplitude…

The lifetimes of the photoluminescent compounds contained in the coating of fluorescent compact lamps are usually measured using specialised instruments, including pulsed lasers and/or spectrofluorometers. Here we discuss how some low cost apparatuses, based on the use of either sensors for the educational lab or commercial digital photo cameras,…

This laboratory introduces students to laser-induced breakdown spectroscopy (LIBS) for the analysis of metals in soil and rock samples. LIBS employs a laser-initiated spark to induce electronic excitation of metal atoms. Ensuing atomic emission allows for qualitative and semiquantitative analysis. The students use LIBS to analyze a series of…

This laboratory experiment introduces students to a different type of atomic spectroscopy: laser-induced breakdown spectroscopy (LIBS). LIBS uses a laser-generated spark to excite the sample; once excited, the elemental emission is spectrally resolved and detected. The students use LIBS to analyze a series of standard synthetic silicate samples…

Gives upper level chemistry students a better understanding of the application of lasers in the field of spectroscopy. Provides three experiments to demonstrate the thermal lens effect: determination of beam profile, cell position optimization, and pKa determination. (MVL)