We created a novel laboratory experience where undergraduate students explore the techniques used to study protein misfolding, unfolding, and aggregation. Despite the importance of protein misfolding and aggregation diseases, protein unfolding is not typically explored in undergraduate biochemistry laboratory classes. Yeast alcohol dehydrogenase…

The study of energy metabolism is fundamental in multiple disciplines, such as biochemistry, bioengineering, biomedicine, etc. Traditionally, in high school and college level courses, students are taught energy metabolism (glycolysis, Krebs cycle, and oxidative phosphorylation) through diagrams of metabolic pathways that depict the transformation…

This review extends on previously reported findings in which students were asked to construct Reaction Coordinate Diagrams (RCDs) de novo. Instead of asking students to construct RCDs, this study explores students' understanding of kinetics and thermodynamics when provided with mechanisms for the Aldol and Claisen condensations. Data was collected…

The study and analysis of enzymatic reactions are essential for characterizing various biological systems. As part of these procedures, it is important to understand how to quantify the biological activity of these proteins in terms of enzymatic activity. This work proposes a general equation for determining enzymatic activity based on…

Most textbooks and lecturers present Michaelis-Menten kinetics using the equation v = V[subscript max][S]/(K[subscript m] + [S]). There are advantages to presenting this relationship in a slightly different form, namely v = V[subscript max]/{1 + (K[subscript m]/[S])}. We articulate advantages for single-substrate reactions and extend the formalism…

Energy conversion devices such as fuel cells, metal-air batteries, and electrolyzers have been envisaged as possible solutions for cutting down the continuous accumulation of greenhouse gases resulting from the combustion of fossil fuel. The bottleneck reaction for these devices is the oxygen reduction reaction (ORR) occurring at the cathode. The…

This article aims to simplify and facilitate the process of practical teaching of enzyme kinetics by utilizing minimal teaching laboratory requirements. Simultaneously, it ensures that students comprehend the enzyme kinetics experiment effectively. The focus is on teaching students how to estimate the maximum velocity (Vmax) and Michaelis constant…

"k[subscript cat]/K[subscript M]" is a fundamental kinetic parameter as important as "k[subscript cat]" in enzyme kinetics. Although the parameter provides critical information on how the enzyme lowers the energy barriers along the reaction pathway for catalysis, "k[subscript cat]/K[subscript M]" is frequently…

Chemical reactions that mimic the function of ATP hydrolysis in biochemistry are of current interest in nonequilibrium systems chemistry. The formation of transient bonds from these reactions can drive molecular machines or generate materials with time-dependent properties. While the behavior of these systems can be complicated, the underlying…

Teaching special relativity to lower-division students is challenging because results such as time dilation, length contraction, and frame dependence of simultaneity are counterintuitive. The literature is extensive, so only a brief list is given here, with articles divided roughly between discussions of general principles and calculations applied…

Scientific reasoning competencies enable students to engage in scientific inquiry through a wide range of methods, for example by asking questions, formulating hypotheses, and planning, carrying out, and evaluating experiments. Laboratories are particularly suitable to foster these competencies in higher education. In order to give lab educators…

Enzymes are nature's catalysts, mediating chemical processes in living systems. The study of enzyme function and mechanism includes defining the maximum catalytic rate and affinity for substrate/s (among other factors), referred to as enzyme kinetics. Enzyme kinetics is a staple of biochemistry curricula and other disciplines, from molecular and…

A loop-the-loop experiment usually involves a ball rolling around a vertical loop. A different version of the experiment is described where a nut was allowed to slide around a vertical loop. In both experiments there is a large decrease in kinetic energy when the ball or the nut first enters the loop.

A collision of one object with two or more objects is relatively complicated in general, but a simple example is provided by Newton's cradle since all the objects are identical and in line. In the present paper, an experiment is described where a heavy mallet collides head-on with two billiard balls. The two conservation equations indicate that…

Races between moving objects are an engaging way to teach dynamics to introductory physics students. One standard example consists in racing hollow and solid cylinders as they roll down an inclined plane. Another striking demonstration is a race between two marbles on side-by-side tracks that start and end together, but with one track taking a…