Biochemistry is an important professional course to undergraduates majoring in rehabilitation therapy in medical colleges and universities. To deepen students' understanding of the taught content, enhance their application ability and cultivate their high-level thinking ability, we investigated the effect of integration of the nine-grid thinking model into the teaching process. With the inline and divergence of relevant knowledge as the guiding ideology, students' understanding of knowledge points was deepened through thinking visualization. According to the questionnaire survey, 75% of the students believed that the application of the nine-grid thinking model was an effective teaching method for improving the efficiency of teaching and enhancing the teaching effect. In addition, a team of four students from the investigated class were granted by the 2021 Shanxi University Student Innovation and Entrepreneurship Training Program and awarded RMB 6000 as a research fund (20210563). According to them, the application of the nine-grid thinking model in teaching is of great significance for cultivating students' higher-order thinking ability. The findings of this study might provide a new, effective approach to college course teaching.