This undergraduate laboratory experiment integrates multiple techniques ("in vitro" synthesis, enzyme assays, Western blotting) to determine the production and detection sensitivity of two common reporter proteins (beta-galactosidase and luciferase) within an "Escherichia coli" S30 transcription/translation extract. Comparison of the data suggests…

An experimental exercise on analytical proficiency studies in collaborative trials is proposed. This practical provides students in advanced undergraduate courses in chemistry, pharmacy, and biochemistry, with the opportunity to improve their quality assurance skills. It involves an environmental analysis, determining the concentration of a…

An exciting laboratory environment is activated by the preparation and novel use of magnetic materials to decontaminate water through adsorption and magnetic removal of metals and organics. This uncomplicated technique is also adaptable to the possible application of adsorbents to numerous other environmental substances.

Green chemistry is the utilization of a set of principles that reduces or eliminates the use or generation of hazardous substances in the design, manufacture and application of chemical products. Some of the philosophical questions and practical decisions that have guided the greening of the organic chemistry laboratory at Hendrix College in…

The potassium salt is an easy product to synthesize in an introductory course on inorganic chemistry and the students are required to prepare this product in order to improve their laboratory skills and as an introduction to the synthesis of coordination compounds. The complex potassium tris (oxalato) ferrate (III) is used to illustrate the…

The Kirby-Bauer method is regularly used to test bacterial susceptibility to antibiotics, and is often employed in the classroom for teaching this concept. In this exercise, additional materials and instructions were given to students for the preparation of garlic extract and loading on blank BBL paper discs. They were further instructed to test…

One easy way to reduce the number of accidents in the lab is to go "green." Green chemistry, or sustainable chemistry, emerged about a decade ago, but the concept has been practiced for centuries by indigenous people of many continents. The basic principles of green chemistry are that you should use only what you need and recycle what you can.…

A three-hour laboratory experiment, designed for an advanced undergraduate course in instrumental analysis that illustrates the application of the gradient chamber flow-injection titration (GCFIT) method with spectrophotometric detection to determine acidity constants is presented. The procedure involves the use of an acid-base indicator to obtain…

It is challenging for biomedical engineering programs to incorporate an indepth study of the systemic interdependence of cells, tissues, and organs into the rigorous mathematical curriculum that is the cornerstone of engineering education. To be sure, many biomedical engineering programs require their students to enroll in anatomy and physiology…

Describes an undergraduate chemistry experiment in which the concentration of chloride, fluoride, or bromide ions can be quantitatively determined by titrating with mercury (II) ions. (MLH)

A reactor to convert waste rubber tires to useful products such as CO and H2, was investigated in a university undergraduate design project. The student worked individually with mentorship from a faculty professor who aided the student with professional critique. The student was able to research the background of the field and conceive of a novel…

A low-cost tensile testing machine was built for testing polymeric films. This apparatus also allows for tear-strength and flexural tests. The experimental results, obtained from common-use materials, selected by the students, such as plastic bags, illustrate important aspects of the mechanical behavior of polymeric materials. Some of the tests…