A laboratory experiment introducing the concept of chemical bioconjugation of proteins to undergraduate students in a therapeutically relevant context was developed. Initially, students installed an aldehyde functionality into a protein via the oxidation of the N-terminal threonine residue of the cholera toxin subunit B (CTB) protein, which was…

A laboratory experiment was developed to investigate the rheological properties of a green calcium-cross-linked alginate gel as an alternative to the traditional borax-cross-linked poly(vinyl alcohol) gel. As borax is suspected of damaging fertility and the unborn child, a safe, green alternative is necessary. The rheological properties of a…

A laboratory experiment was developed to enable students to investigate the use of smart hydrogels for potential application in targeted drug delivery. This is challenging for students to explore practically because of the extremely high risks of handling cross-linking agents such as glutaraldehyde. Genipin is a safe and green alternative that has…

A laboratory experiment was developed to follow the gelation of a polyvinyl alcohol (PVA) solution upon addition of borax by using rotational viscometry. The rheological properties of the gel were examined, measuring the dependence of viscosity and shear stress on the shear rate. Time-dependent studies were also conducted in which the viscosity of…