We created a novel laboratory experience where undergraduate students explore the techniques used to study protein misfolding, unfolding, and aggregation. Despite the importance of protein misfolding and aggregation diseases, protein unfolding is not typically explored in undergraduate biochemistry laboratory classes. Yeast alcohol dehydrogenase…

The inhibition of porcine pancreatic lipase by the drug orlistat was studied through a series of activities that combine experimental and computational procedures. The enzymatic activity of the lipase was determined by measuring enzyme kinetics and calculating parameters such as K[subscript m] and V[subscript max] from experimental data. Assays of…

Water pollution has been one of the major issues of the present time. It is essential to educate students regarding this issue and its possible solutions. In this article, a demonstration of a typical adsorption-based water cleaning process is presented. The removal of methylene blue (MB) and detergent from an aqueous solution is demonstrated…

In this paper we present a novel approach to teach kinetic energy as an observable and measurable concept. The fundamental principles have been outlined for gravitational potential energy in an earlier work, that we extend here. By accelerating or decelerating bodies with springs we begin, in a first step, with a direct comparison method for…

The so-called Diet Coke and Mentos experiment is initiated by dropping Mentos candies into a bottle of Diet Coke or other carbonated beverage. This causes the beverage to rapidly degas, causing foam to stream out of the bottle. Simple application of the gas laws leads to the straightforward prediction that ejection of greater foam volume is…

Many studies have examined the structure and properties of the Force Concept Inventory (FCI); however, far less research has investigated the Force and Motion Conceptual Evaluation (FMCE). This study applied Multidimensional Item Response Theory (MIRT) to a sample of N = 4528 FMCE post-test responses. Exploratory factor analysis showed that 5, 9,…

A novel method of measuring the coefficients of kinetic and rolling friction is proposed. The method is simple to implement and reliable. Samples of measurements and calculations are presented.

A magnetic linear accelerator (or Gauss accelerator) is a device that uses the conversion of magnetic energy into kinetic energy to launch an object with high velocity. A simple experimental implementation consists of a line of steel spheres in which the first one is a permanent magnetic sphere. If another steel ball collides with the magnetic…

The cannon boring experiment of Count Rumford, where eight kilograms of water were boiled by metal on metal friction, is investigated. Consideration of this dramatic demonstration can enrich classroom discussions of calorimetry, units of measure, elements, and thermodynamics. A section pertaining to use of the article in the classroom appears…

In selecting from a repertoire of traditional kinetics experiments, an instructor often has to choose among having students gain experience with the graphical method, the method of initial rates, or a temperature-dependent experiment in which students construct an Arrhenius plot. This paper presents an environmentally friendly bleaching reaction…

A cordless mouse with an added reed switch is used as a wireless data logger to record every time the wheel of a trolley completes a revolution. The limitations of the system in terms of maximum clicking rate and spatial resolution are considered and data obtained from the descent of a trolley down a ramp at various different angles is analysed in…

The effect of the aerodynamic drag force on an object in flight is well known and has been described in this and other journals many times. At speeds less than about 1 m/s, the drag force on a sphere is proportional to the speed and is given by Stokes' law. At higher speeds, the drag force is proportional to the velocity squared and is…

Measurements are presented on the rise of a spinning egg. It was found that the spin, the angular momentum and the kinetic energy all decrease as the egg rises, unlike the case of a ballerina who can increase her spin and kinetic energy by reducing her moment of inertia. The observed effects can be explained, in part, in terms of rolling friction…

The synthesis of N-methoxycarbonyl-2-imidazolidone, an analogue of biotin, was conducted by organic chemistry students and confirmed using FT-IR and H NMR. Spectroscopy students used FT-IR to measure the rate of hydrolysis of the product and determined the rate constant for the reaction using the integrated rate law. From the magnitude of the rate…

We have recently designed a biochemistry laboratory experiment for the purpose of providing students an advanced experience with enzyme kinetics and the kinetics of binding. Bestatin, a well-known and commercially available general protease inhibitor, is a slow-binding inhibitor of aminopeptidase isolated from "Aeromonas proteolytica." The binding…