With the enormous reliance on plastics in the world today, bisphenol A, an additive used in the synthesis of many plastics, such as polycarbonate, has been found almost everywhere in our environment including in soil, in natural waters, and inside organisms including humans. A multidisciplinary experiment is presented that involves using flatworms…

Food safety science is an important field due to its practical applications in maintaining public safety and confidence in consumer goods. A significant component of food safety science is the detection and regulation of heavy metals in food. Heavy metals such as mercury (Hg) are of particular concern because of their potential to damage the…

Polychlorinated biphenyls (PCBs) are a family of synthetic chemicals consisting of carbon, hydrogen, and chlorine atoms. PCBs have been produced commercially since 1929 in hundreds of industrial applications. Their continued production in the United States was phased out in the late 1970s, and strict disposal guidelines were regulated as adverse…

A team of six children (13-14 years old) developed and conducted an experiment to assess the behaviour of the planarian flatworm, an invertebrate animal model, before, during and after exposure to chemicals. The aim of the project was to engage children in pharmacology and toxicology research. First, the concept that exposure to chemicals can…

A laboratory experiment is described that extracts the tetrapyrrolic teal-colored biliverdin IXa, as its dimethyl ester, from commercially available emu eggshells. The extraction of ~10 mg samples of biliverdin is simple and requires two 3 h lab periods: A two-step acid digestion and liquid-liquid extraction, followed by short silica gel flash…

A series of three practical sessions are designed to give students firsthand experience with the preparation of natural product extracts and assay using a live tissue preparation. Areca or betel nuts are the seeds from the fruit of the "Areca catechu" palm tree that is known to contain a number of pharmacologically active alkaloids. The…

Analysis of mercury in fish is an interesting problem with the potential to motivate students in chemistry laboratory courses. The recommended method for mercury analysis in fish is cold vapor atomic absorption spectroscopy (CVAAS), which requires homogeneous analyte solutions, typically prepared by acid digestion. Previously published digestion…

Nanotoxicity is an area of intense research, stimulated by increased use of nanoparticles in commercially available products. Herein, using nanotoxicity as a platform, we describe an experiment that emphasizes interdisciplinary science in a collaborative work setting while expanding the traditional realm of chemistry and chemistry research.…

Mercury is a known neurotoxin that is particularly harmful to children and unborn fetuses. Consumption of contaminated fish is one major route of mercury exposure. This laboratory experiment gives students an opportunity to measure mercury concentrations in store-bought seafood and compare the results to suggested exposure limits. The U.S.…

Anaerobic metabolism is recruited in vertebrates under conditions of intense exercise or lowered environmental oxygen availability (hypoxia), typically resulting in the accumulation of lactate in blood and tissues. Lactate will be cleared over time after the reoxygenation of tissues, eventually returning to control levels. Here, we present a…

The surface-to-volume ratio is an important quantity in thermal physics. For example it governs the behaviour of heating or cooling of physical objects as a function of size like, e.g. cubes or spheres made of different material. The starting point in our paper is the simple physics problem of how cheese cubes of different sizes behave if heated…

An experiment is conducted to ascertain trace-level Pb in samples of bovine liver or muscle by applying graphite furnace atomic absorption spectrophotometry (GFAAS). The primary objective is to display the effects of physical and spectral intrusions in determining trace elements, and project the usual methods employed to minimize accuracy errors…

A laboratory experiment that provides students a hands-on introduction to the specific techniques of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and electrospray ionization is presented. The students can thus practice the analytical principles of sample extraction, detection, quantification, and quality control using a fresh fish…

We present a two-part undergraduate laboratory exercise. In the first part, electron transport in bovine heart submitochondrial particles causing reduction of cytochrome c is monitored at 550 nm. Redox-active dyes have historically been used in most previous undergraduate laboratory exercises of this sort but do not demonstrate respiratory…