The Förster resonance energy transfer (FRET) melting assay intends to evaluate the unfolding, denaturation process of DNA secondary structures, and its stabilization using compounds known as DNA binders, some of which are highly specific for G-quadruplex DNAs versus duplex DNAs. First, students determined the melting temperature (T[subscript m])…

Next generation sequencing has revolutionized molecular biology and has provided a mechanism for rapid DNA and RNA sequence analysis. Yet, there are few resources to introduce next generation sequencing into the undergraduate biochemistry and molecular biology curriculum. Herein, we describe the design, execution, and assessment of a four-week…

Demonstration of a colorimetric approach for nucleic acid detection represents an attractive educational experiment for chemistry undergraduate students in the time of coronavirus pandemic. Herein, a rapid and vivid detection method that visualizes the presence of a specific DNA sequence is described. The plasmon resonance of gold nanoparticles…

Both nanopore-based DNA sequencing and CRISPR/Cas-based gene editing represent groundbreaking innovations in molecular biology and genomics, offering unprecedented insights into and tools for working with genetic information. For students, reading, editing, and even writing DNA will be part of their everyday life. We have developed a laboratory…

The field of DNA nanotechnology has grown rapidly in the past decade and has expanded to multiple laboratories. While lectures in DNA nanotechnology have been introduced in some institutions, laboratory components at the undergraduate level are still lacking. Undergraduate students predominantly learn about DNA nanotechnology through their…

Structure--activity relationships are foundational concepts in biochemistry and drug design. Despite their importance, it can be difficult to create and execute laboratory activities that allow students to directly observe the interactions between small-molecule drugs and their biomolecular targets. Here, we report an activity that investigates…

The CRISPR/Cas9 system is a powerful tool for gene editing and it has become increasingly important for biology students to understand this emerging technique. Most CRISPR laboratory teaching modules use complex metazoan systems or mammalian cell culture which can be expensive. Here, we present a lab module that engages students in learning the…

The COVID-19 pandemic has forced the Bioinformatics course to switch from on-site teaching to remote learning. This shift has prompted a change in teaching methods and laboratory activities. Students need to have a basic understanding of DNA sequences and how to analyze them using custom scripts. To facilitate learning, we have modified the course…

We used a novel approach that combined direct PCR of plant tissue and SRAP DNA fingerprinting to seek to identify the crosses made in "Brassica rapa" used in the Wisconsin Fast Plants. Our goal was to develop a rapid method for students to use in a biology, botany, or genetics class to identify the parental types (purple stem, non-purple…

CRISPR is a technique increasingly used in the laboratory for both fundamental and applied research. We designed and implemented a lab experience for undergraduates to carry out CRISPR technology in the lab, and knockout the wing patterning genes "optix" and "WntA" in "Vanessa cardui" butterflies. Students obtained…

An experiment for the upper-division undergraduate biochemistry laboratory is described in which students investigate the influence of genetic variations of cytochrome P450 1A2 on drug metabolism, using caffeine as a model compound. Saliva samples from human subjects are characterized for a single-nucleotide polymorphism in the CYP1A2 gene…

Disrupting a gene to determine its effect on an organism's phenotype is an indispensable tool in molecular biology. Such techniques are critical for understanding how a gene product contributes to the development and cellular identity of organisms. The explosion of genomic sequencing technologies combined with recent advances in genome-editing…

Categorizing the various levels of protein and nucleic acid structures is a relatively straightforward concept students understand quickly when shown representative structural images. However, keeping in mind the idea of primary, secondary, and tertiary structure proves more difficult when students are in the laboratory setting simply looking at a…

CRISPR-Cas9 is revolutionizing how we conduct scientific research, treat disease, and develop new crops. The widespread impact of this genome-editing technology makes it critical for undergraduate students to understand and engage with this new tool. In this article, we describe a multi-week lab module that teaches undergraduates how to design…

A bioinformatics laboratory experiment was designed to help undergraduate students learn RNA structure and function in biochemistry courses. In this laboratory experiment, students used 5S rRNAs as material to predict secondary structure and visualize three-dimensional (3D) structure. Students first downloaded molecular information from the 5S…